• Product NameMUC1 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.SN06-80
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IHC, IP, FC
• Species ReactivityHu
• Immunogen Descriptionrecombinant protein
• Alternative Names    ADMCKD antibody
  ADMCKD1 antibody
  Breast carcinoma associated antigen DF3 antibody
  Breast carcinoma-associated antigen DF3 antibody
  CA 15-3 antibody
  CA15 3 antibody
  CA15 3 antigen antibody
  CA15.3 antibody
  Cancer antigen 15-3 antibody
  Carcinoma associated mucin antibody
  Carcinoma-associated mucin antibody
  CD 227 antibody
  CD227 antibody
  DF3 antigen antibody
  EMA antibody
  Episialin antibody
  H23 antigen antibody
  H23AG antibody
  KL 6 antibody
  KL-6 antibody
  KL6 antibody
  Krebs von den Lungen-6 antibody
  MAM 6 antibody
  MAM6 antibody
  MCD antibody
  MCKD antibody
  MCKD1 antibody
  Medullary cystic kidney disease 1 (autosomal dominant) antibody
  Medullary cystic kidney disease, autosomal dominant antibody
  MUC 1 antibody
  MUC-1 antibody
  MUC-1/SEC antibody
  MUC-1/X antibody
  MUC1 antibody
  MUC1-alpha antibody
  MUC1-beta antibody
  MUC1-CT antibody
  MUC1-NT antibody
  MUC1/ZD antibody
  MUC1_HUMAN antibody
  Mucin 1 antibody
  Mucin 1 transmembrane antibody
  Mucin 1, cell surface associated antibody
  Mucin-1 subunit beta antibody
  Peanut reactive urinary mucin antibody
  Peanut-reactive urinary mucin antibody
  PEM antibody
  PEMT antibody
  Polymorphic epithelial mucin antibody
  PUM antibody
  Tumor associated epithelial membrane antigen antibody
  Tumor associated epithelial mucin antibody
  Tumor associated mucin antibody
  Tumor-associated epithelial membrane antigen antibody
  Tumor-associated mucin antibody
  
• Accession No. Swiss-Prot#:P15941
• UniprotP15941
• Gene ID4582;
• Calculated MW 23 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-1:2,000
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  
  FC: 1:50-1:100

The mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (Mucin 1), 69 base pairs (Mucin 2) and 51 base pairs (Mucin 3). The number of repeats is highly polymorphic and varies among different alleles. Mucin 1 proteins are expressed as type I membrane proteins in addition to secreted forms. Mucin 1 is aberrantly expressed in epithelial tumors including breast carcinomas. Mucin 2 coats the epithelia of the intestines and airways and is associated with colonic tumors. Mucin 3 is a major com-ponent of various mucus gels and is broadly expressed in normal and tumor cells.

• Western blot analysis of MUC1 on Hela cells lysates using anti-MUC1 antibody at 1/1,000 dilution.
• Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-MUC1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MUC1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MUC1 antibody. Counter stained with hematoxylin.
• ICC staining MUC1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining MUC1 in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of MCF-7 cells with MUC1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting