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SOX9 Antibody

49070
Review: comment rank 5 pdf
  Catalog No. Package Size Price
49070-1 50ul $260.00
49070-2 100ul $390.00

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Description
  • Product Name SOX9 Antibody
  • Clone No. SN74-09
  • Purification ProA affinity purified
  • Applications WB, ICC/IF, IHC, IP, FC
  • Species Reactivity Hu, Ms, Rt
  • Immunogen Description recombinant protein
  • Alternative Names campomelic dysplasia autosomal sex reversal antibody
    CMD 1 antibody
    CMD1 antibody
    CMPD 1 antibody
    CMPD1 antibody
    SOX 9 antibody
    Sox9 antibody
    SOX9_HUMAN antibody
    SRA 1 antibody
    SRA1 antibody
    SRXX2 antibody
    SRXY10 antibody
    SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal antibody
    SRY (sex determining region Y) box 9 antibody
    SRY (sex determining region Y)-box 9 antibody
    SRY (sex-determining region Y)-box 9 protein antibody
    SRY related HMG box gene 9 antibody
    Transcription factor SOX 9 antibody
    Transcription factor SOX-9 antibody
    transcription factor SOX9 antibody
  • Accession No. Swiss-Prot#:P48436
  • Calculated MW 56 kDa
  • Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage Store at -20˚C
Application Details

WB: 1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100

Images
  • SOX9 Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SOX9 antibody. Counter stained with hematoxylin.

  • SOX9 Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-SOX9 antibody. Counter stained with hematoxylin.

  • SOX9 Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SOX9 antibody. Counter stained with hematoxylin.

  • SOX9 Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-SOX9 antibody. Counter stained with hematoxylin.

  • SOX9 Antibody - SAB | Signalway Antibody

    ICC staining SOX9 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • SOX9 Antibody - SAB | Signalway Antibody

    ICC staining SOX9 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • SOX9 Antibody - SAB | Signalway Antibody

    Flow cytometric analysis of Hela cells with SOX9 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Background

Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at least 40 different loci that rapidly diverged in various animal lineages. At present, 30 Sox genes have been identified. Members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal.

Regerences

1. Winkler T et al. Wnt signaling activates Shh signaling in early postnatal intervertebral discs, and re-activates Shh signaling in old discs in the mouse. PLoS One 9:e98444 (2014).
2. Yamamizu K et al. SOX9 accelerates ESC differentiation to three germ layer lineages by repressing SOX2 expression through P21 (WAF1/CIP1). Development 141:4254-66 (2014).

    Please let us know if you have published research using #49070 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
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