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RAB7 Antibody

49113
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  Catalog No. Package Size Price
49113-1 50ul $260.00
49113-2 100ul $390.00

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Description
  • Product Name RAB7 Antibody
  • Clone No. SN202-03
  • Purification ProA affinity purified
  • Applications WB, ICC/IF, IHC, FC
  • Species Reactivity Hu, Ms, Rt
  • Immunogen Description recombinant protein
  • Alternative Names CMT2B antibody
    PRO2706 antibody
    PSN antibody
    RAB7, member RAS oncogene family antibody
    RAB7A antibody
    RAB7A, member RAS oncogene family antibody
    RAB7A_HUMAN antibody
    Ras associated protein RAB7 antibody
    Ras related protein Rab7 antibody
    Ras related protein Rab7a antibody
    Ras-related protein Rab-7a antibody
  • Accession No. Swiss-Prot#:P51149
  • Calculated MW 23 kDa
  • Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage Store at -20˚C
Application Details

WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:100-1:500

FC: 1:50-1:100

Images
  • RAB7 Antibody - SAB | Signalway Antibody

    Flow cytometric analysis of K562 cells with RAB7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

  • RAB7 Antibody - SAB | Signalway Antibody

    ICC staining RAB7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • RAB7 Antibody - SAB | Signalway Antibody

    ICC staining RAB7 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • RAB7 Antibody - SAB | Signalway Antibody

    ICC staining RAB7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • RAB7 Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-RAB7 antibody. Counter stained with hematoxylin.

  • RAB7 Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-RAB7 antibody. Counter stained with hematoxylin.

  • RAB7 Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-RAB7 antibody. Counter stained with hematoxylin.

  • RAB7 Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-RAB7 antibody. Counter stained with hematoxylin.

  • RAB7 Antibody - SAB | Signalway Antibody

    Western blot analysis of RAB7 on different lysates using anti-RAB7 antibody at 1/1,000 dilution. Positive control:
    Lane 1: A431
    Lane 2: C2C12

Background

The Ras-related superfamily of guanine nucleotide binding proteins, which includes the Ral/Rec, Rap, R-Ras, and Rho/Rab subfamilies, exhibit 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the Sec4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles. Several members of the Rab subfamily have been identified, each of which is found at a particular stage of a membrane transport pathway.

Regerences

1. Fields J et al. HIV-1 Tat alters neuronal autophagy by modulating autophagosome fusion to the lysosome: implications for HIV-associated neurocognitive disorders. J Neurosci 35:1921-38 (2015).
2. Kanagaraj P et al. Souffle/Spastizin Controls Secretory Vesicle Maturation during Zebrafish Oogenesis. PLoS Genet 10:e1004449 (2014).

    Please let us know if you have published research using #49113 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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