• Product NamePRMT1 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JA11-17
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IHC, CHIP
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• Alternative Names    ANM 1 antibody
  ANM1 antibody
  ANM1_HUMAN antibody
  HCP 1 antibody
  HCP1 antibody
  Heterogeneous nuclear ribonucleoprotein methyltransferase 1 like 2 antibody
  Heterogeneous nuclear ribonucleoproteins methyltransferase like 2 antibody
  Heterogeneous nuclear ribonucleoproteins methyltransferase like2 antibody
  Histone-arginine N-methyltransferase PRMT1 antibody
  HMT 2 antibody
  HMT1 (hnRNP methyltransferase S. cerevisiae) like 2 antibody
  HMT1 hnRNP methyltransferase antibody
  HMT1 hnRNP methyltransferase like 2 (S. cerevisiae) antibody
  HMT1 hnRNP methyltransferase like 2 antibody
  HMT2 antibody
  HRMT1 L2 antibody
  HRMT1L 2 antibody
  HRMT1L2 antibody
  Human mRNA for suppressor for yeast mutant antibody
  Human mRNA for suppressor for yeast mutant complete cds antibody
  Interferon receptor 1 bound protein 4 antibody
  Interferon receptor 1 bound protein4 antibody
  Interferon receptor 1-bound protein 4 antibody
  Interferon receptor 1bound protein 4 antibody
  IR1 B4 antibody
  IR1B 4 antibody
  IR1B4 antibody
  Mrmt 1 antibody
  Mrmt1 antibody
  PRMT 1 antibody
  PRMT1 antibody
  Protein arginine methyltransferase 1 antibody
  Protein arginine N methyltransferase 1 antibody
  Protein arginine N methyltransferase1 antibody
  Protein arginine N-methyltransferase 1 antibody
  R1B4 antibody
  S. cerevisiae like 2 antibody
  
• Accession No. Swiss-Prot#:Q99873
• UniprotQ99873
• Gene ID3276;
• Calculated MW 42 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:1,000
  IHC: 1:50-1:200
  ICC: 1:100-1:500
  
  

A class of proteins termed type 1 protein arginine N-methyltransferase (PRMT) enzymes contribute to posttranslational modification of RNA-binding proteins, but differ in substrate specificities, oligomerization properties and subcellular localization. PRMT1, the predominant form in mammalian cells, is located in the nucleus, while PRMT3 is present in the cytoplasm. At the carboxy-terminus, interleukin enhancer-binding factor 3 (ILF3) binds PRMT1, thereby regulating PRMT1 activitiy. Alternative mRNA splicing of the PRMT gene results in three isoforms of PRMT1 that differ in their amino-terminus regions. All three splice variants of PRMT1 are enzymatically active. PRMT3 recognizes and binds to RNA-associated substrates with a zinc-finger domain in its amino-terminus. The zinc-liganded form of this domain is required for the enzyme to recognize RNA-associated substrates.

• Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-PRMT1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-PRMT1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PRMT1 antibody. Counter stained with hematoxylin.
• ICC staining PRMT1 in D3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining PRMT1 in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining PRMT1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining PRMT1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining PRMT1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting