• Product NameNFAT2 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JA81-03
• PurificationProA affinity purified
• ApplicationsWB, ICC, IHC, FC
• Species ReactivityHu
• Immunogen Descriptionrecombinant protein
• Alternative Names    cytoplasmic 1 antibody
  MGC138448 antibody
  NF ATc antibody
  NF ATc1 antibody
  NF-ATc antibody
  NF-ATc1 antibody
  NF-ATc1.2 antibody
  NFAC1_HUMAN antibody
  NFAT 2 antibody
  NFAT transcription complex cytosolic component antibody
  NFATC 1 antibody
  NFATc antibody
  NFATc1 antibody
  Nuclear factor of activated T cells cytoplasmic 1 antibody
  Nuclear factor of activated T cells cytoplasmic calcineurin dependent 1 antibody
  Nuclear factor of activated T cells cytosolic component 1 antibody
  nuclear factor of activated T-cells 'c' antibody
  Nuclear factor of activated T-cells antibody
  
• Accession No. Swiss-Prot#:O95644
• UniprotO95644
• Gene ID4772;
• Calculated MW 101 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• 
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  
  FC: 1:50-1:100

Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells. Required for osteoclastogenesis and regulates many genes important for osteoclast differentiation and function.

• Immunohistochemical analysis of paraffin-embedded human thymus tissue using anti-NFAT2 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NFAT2 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NFAT2 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human skeleton muscle tissue using anti-NFAT2 antibody. Counter stained with hematoxylin.
• ICC staining NFAT2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining NFAT2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining NFAT2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of Jurkat cells with NFAT2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting