• Product NameDSG1 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JM54-19
• PurificationProA affinity purified
• ApplicationsWB, ICC, IF, IHC-P
• Species ReactivityHuman, Mouse, Rat, Zebrafish
• Immunogen DescriptionSynthetic peptide within N-terminal human Aldolase
• Alternative Names    Cadherin family member 4 antibody
  CDHF4 antibody
  Desmoglein-1 antibody
  Desmosomal glycoprotein 1 antibody
  DG1 antibody
  DGI antibody
  DSG antibody
  DSG1 antibody
  DSG1_HUMAN antibody
  EPKHE antibody
  EPKHIA antibody
  Pemphigus foliaceus antigen antibody
  PPKS1 antibody
  SPPK1 antibody
  
• Accession No. Swiss-Prot#:Q02413
• UniprotQ02413
• Gene ID1828;
• Calculated MW 39 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:2,000

  ICC/IF: 1:50-1:200

  IHC-P:1:50-1:200

   

Desmogleins (dsgs) are type I membrane proteins that are important for cell adhesion and are expressed in great abundance at the desmosomes, which are adhesive cell junctions. The dsg proteins belong to the cadherin family and consist of dsg1, dsg2 and dsg3. Calcium binds to the putative calcium binding sites at the extracellular N-terminal domain of dsg1, which has cadherin-like repeats. Unlike normal human keratinocytes, the squamous cell carcinoma cells exhibit diminished or unusual expression of dsg3 and dsg1, which bear pemphigus vulgaris and pemphigus foliaceus antigens, respectively. Cultured normal human keratinocytes express dsg1 and dsg3 mRNA, with or without dsg 2 mRNA, which indicates that desmoglein isoforms exhibit abnormal expression and may be related to tumor cell kinetics, such as cell invasion and metastasis. Pemphigus is an autoimmune disease of skin adhesion associated with auto-antibodies against a number of keratinocyte antigens, such as the adhesion molecules dsg 1 and 3 and acetylcholine receptors.

• Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-DSG1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-DSG1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-DSG1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-DSG1 antibody. Counter stained with hematoxylin.
• Western blot analysis of Aldolase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: A549 cell lysateLane 2: Hela cell lysateLane 3: Mouse liver tissue lysateLane 4: Rat liver tissue lysateLane 5: Rat spleen tissue lysate
• Western blot analysis of Aldolase on zebrafish tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.
• ICC staining of Aldolase in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
• ICC staining of Aldolase in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting