• Product NamePhospho-Creb (S133) Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• Clone No.JB25-40
• PurificationProA affinity purified
• ApplicationsWB,ICC,IF,IHC,IP,FC
• Species ReactivityHu, Ms
• Immunogen DescriptionSynthetic phospho-peptide corresponding to residues surrounding Ser133 of human Creb.
• Alternative Names    Active transcription factor CREB antibody
  cAMP response element binding protein 1 antibody
  cAMP response element binding protein antibody
  cAMP responsive element binding protein 1 antibody
  cAMP-responsive element-binding protein 1 antibody
  CREB antibody
  CREB-1 antibody
  CREB1 antibody
  CREB1_HUMAN antibody
  Cyclic AMP-responsive element-binding protein 1 antibody
  MGC9284 antibody
  OTTHUMP00000163864 antibody
  OTTHUMP00000163865 antibody
  OTTHUMP00000206660 antibody
  OTTHUMP00000206662 antibody
  OTTHUMP00000206667 antibody
  Transactivator protein antibody
  
• Accession No. Swiss-Prot#:P16220
• UniprotP16220
• Gene ID1385;
• Calculated MW Predicted band size 37 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:2,000
  IHC: 1:50-1:200
  ICC: 1:50-1:100
  IP: 1:10-1:50
  FC: 1:50-1:100

Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.

• Western blot analysis of Phospho-Creb (S133) on human colon tissue lysates using anti-Phospho-Creb (S133) antibody at 1/500 dilution.
• Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Phospho-Creb (S133) antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Phospho-Creb (S133) antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Phospho-Creb (S133) antibody. Counter stained with hematoxylin.
• ICC staining Phospho-Creb (S133) in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Phospho-Creb (S133) in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of HUVEC cells with Phospho-Creb (S133) antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting