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Mouse Anti-Human CD274 (PD-1), FITC Conjugated mAb

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  Catalog No. Package Size Price
CM028-1 25T $289.00
CM028-2 100T $609.00

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  • Product Name Mouse Anti-Human CD274 (PD-1), FITC Conjugated mAb
  • Host Species Mouse
  • Clonality Monoclonal
  • Clone No. 2H11
  • Isotype Mouse IgG1, κ
  • Applications FC
  • Species Reactivity Hu
  • Specificity This antibody recognizes human PD-L1 in Western blot and FACS. It cross reacts with mouse PD-L1.
  • Immunogen Description L929/PD-L1 transfected cells
  • Alternative Names Programmed cell death ligand 1 (PD-L1), B7 homolog 1 (B7-H1)
  • Formulation Lyophilized from a 0.2μm filtered solution in phosphate buffered saline (PBS) and reconstitute with sterile PBS .
  • Storage Store protected from light at 2-8˚C. Do not freeze. The expiration date is indicated on the vial label.
Application Details

Preparation:This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with L929/PD-L1 transfected cells). The monoclonal antibody was purified from tissue culture supernatant or ascites by protein G affinity chromatography.
Product Notices:This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 10^6 cells in a 100-?l experimental sample (per test). An isotype control should be used at the same concentration as the antibody of interest.

Product Description
B7-H1 (also known as PD-L1 or CD274) is a B7 family member and it is a ligand for PD-1 (programmed death-1, a member of the CD28 family). PD-L1 is constitutively expressed on a wide variety of immunocytes and nonhematopoietic cell types including some malignant tumors, suggesting the potential mechanism of immune evasion. The enhancement of T cell responses via blockade of PD-1/PD-L1 pathway may be a potential therapeutic strategy for tumor treatment.
    Please let us know if you have published research using #CM028 so that we can cite your reference.
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    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
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    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
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    • Dm Drosophila melanogaster
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