Preparation:This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with L929/PD-L1 transfected cells). The monoclonal antibody was purified from tissue culture supernatant or ascites by protein G affinity chromatography.
Product Notices:This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 10^6 cells in a 100-?l experimental sample (per test). An isotype control should be used at the same concentration as the antibody of interest.

B7-H1 (also known as PD-L1 or CD274) is a B7 family member and it is a ligand for PD-1 (programmed death-1, a member of the CD28 family). PD-L1 is constitutively expressed on a wide variety of immunocytes and nonhematopoietic cell types including some malignant tumors, suggesting the potential mechanism of immune evasion. The enhancement of T cell responses via blockade of PD-1/PD-L1 pathway may be a potential therapeutic strategy for tumor treatment.