• Product NameMouse Anti-Human CD137 (4-1BB), PE Conjugated mAb
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.4A4B4-1
• IsotypeMouse IgG1, κ
• ApplicationsFC
• Species ReactivityHuman, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Squirrel Monkey, Capuchin Monkey, So
• SpecificityThis antibody recognizes human CD137 in Western blot and ELISA.
• Immunogen DescriptionRecombinant human 4-1BB-Fc fusion protein
• Alternative Names    Tumor necrosis factor receptor superfamily, member 9 (TNFRSF9)
• Concentration 5μl per test
• Formulation Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide and 0.2% (w/v) BSA
• Storage Store at 4°C. DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.

• Preparation:This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with recombinant human 4-1BB-Fc fusion protein). The monoclonal antibody was purified from tissue culture supernatant or ascites by protein G affinity chromatography.
  Product Notices:This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 10^6 cells in a 100-?l experimental sample (per test). An isotype control should be used at the same concentration as the antibody of interest.

• CD137 (4-1BB, TNFRSF9) is a surface glycoprotein of the TNFR family which can be induced on a variety of leukocyte subsets, such as activated T cells and NK cells. With the ligation of 4-1BBL, 4-1BB inhibited activation induced cell death, enhanced proliferation and effector functions of T cells.

CDw137 is a 39 kD transmembrane protein also known as 4-1BB. It is expressed on activated T cells. CDw137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily. CDw137 appears to be important for T cell proliferation and survival, and induces monocyte activation through its interaction with 4-1BB ligand. 

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting