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HER2 (Phospho-Tyr1112) Conjugated Antibody

C11903
Review: comment rank 5 pdf
  Catalog No. Package Size Price
C11903-AF350 100ul $379.00
C11903-AF405 100ul $379.00
C11903-AF488 100ul $379.00
C11903-AF594 100ul $379.00
C11903-AF647 100ul $379.00
C11903-AF680 100ul $379.00
C11903-AF750 100ul $399.00
C11903-Biotin 100ul $359.00

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Description
  • Product Name HER2 (Phospho-Tyr1112) Conjugated Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Species Reactivity Hu
  • Specificity The antibody detects endogenous level of HER2 only when phosphorylated at tyrosine 1112.
  • Immunogen Description Peptide sequence around phosphorylation site of tyrosine 1112 (Q-R-Y(p)-S-E) derived from Human HER2.
  • Conjugates Biotin AF350 AF405 AF488 AF594 AF647 AF680 AF750
  • Excitation Emission AF350: 346nm/442nm
    AF405: 401nm/421nm
    AF488: 493nm/519nm
    AF594: 591nm/614nm
    AF647: 651nm/667nm
    AF680: 679nm/702nm
    AF750: 749nm/775nm
  • Alternative Names C-erbB-2;NGL;NEU proto-oncogene;MLN 19
  • Accession No. Swiss-Prot#:P04626
    NCBI Gene ID:2064
    NCBI mRNA#:NM_001005862.2
    NCBI Protein#: NP_001005862.1
  • Calculated MW 150
  • Concentration
  • Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 10mg/ml Bovine Serum Albumin, 0.05% Sodium Azide
  • Storage Store at 4˚Cin dark for 6 months
Application Details

Suggested Dilution:
AF350 conjugated: most applications: 1: 50 - 1: 250
AF405 conjugated: most applications: 1: 50 - 1: 250
AF488 conjugated: most applications: 1: 50 - 1: 250
AF594 conjugated: most applications: 1: 50 - 1: 250
AF647 conjugated: most applications: 1: 50 - 1: 250
AF680 conjugated: most applications: 1: 50 - 1: 250
AF750 conjugated: most applications: 1: 50 - 1: 250
Biotin conjugated: working with enzyme-conjugated streptavidin,most applications: 1: 50 - 1: 1,000

Product Description
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Background
Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-alpha and amphiregulin.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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