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Ezrin (Phospho-Tyr146) Conjugated Antibody

C11909
Review: comment rank 5 pdf
  Catalog No. Package Size Price
C11909-AF350 100ul $379.00
C11909-AF405 100ul $379.00
C11909-AF488 100ul $379.00
C11909-AF594 100ul $379.00
C11909-AF647 100ul $379.00
C11909-AF680 100ul $379.00
C11909-AF750 100ul $399.00
C11909-Biotin 100ul $359.00

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Description
  • Product Name Ezrin (Phospho-Tyr146) Conjugated Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Species Reactivity Hu
  • Specificity The antibody detects endogenous level of Ezrin only when phosphorylated at tyrosine 146.
  • Immunogen Description Peptide sequence around phosphorylation site of tyrosine 146 (S-G-Y(p)-L-S) derived from Human Ezrin.
  • Conjugates Biotin AF350 AF405 AF488 AF594 AF647 AF680 AF750
  • Excitation Emission AF350: 346nm/442nm
    AF405: 401nm/421nm
    AF488: 493nm/519nm
    AF594: 591nm/614nm
    AF647: 651nm/667nm
    AF680: 679nm/702nm
    AF750: 749nm/775nm
  • Alternative Names Cytovillin;EZRI;p81;VIL2;Villin 2
  • Accession No. Swiss-Prot#:P15311
    NCBI Gene ID:7430
    NCBI mRNA#:NM_001111077.1
    NCBI Protein#: NP_001104547.1
  • Calculated MW 69
  • Concentration
  • Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 10mg/ml Bovine Serum Albumin, 0.05% Sodium Azide
  • Storage Store at 4˚Cin dark for 6 months
Application Details

Suggested Dilution:
AF350 conjugated: most applications: 1: 50 - 1: 250
AF405 conjugated: most applications: 1: 50 - 1: 250
AF488 conjugated: most applications: 1: 50 - 1: 250
AF594 conjugated: most applications: 1: 50 - 1: 250
AF647 conjugated: most applications: 1: 50 - 1: 250
AF680 conjugated: most applications: 1: 50 - 1: 250
AF750 conjugated: most applications: 1: 50 - 1: 250
Biotin conjugated: working with enzyme-conjugated streptavidin,most applications: 1: 50 - 1: 1,000

Product Description
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Background
Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
    Please let us know if you have published research using #C11909 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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