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B-Myb (Phospho-Thr494/497) Antibody

12420
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  Catalog No. Package Size Price
12420-1 50ul $189.00
12420-2 100ul $279.00

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Description
  • Product Name B-Myb (Phospho-Thr494/497) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications WB IHC Elisa
  • Species Reactivity Hu Ms Rt
  • Specificity B-Myb (Phospho-Thr494/497) Antibody detects endogenous levels of B-Myb only when phosphorylated at Thr494/497
  • Immunogen Type Peptide
  • Immunogen Description A synthesized peptide derived from human B-Myb (Phospho-Thr494/497)
  • Target Name B-Myb
  • Modification Phospho
  • Alternative Names MYBL2, B-MYB, Myb-like protein 2, Myb-related protein B, BMYB
  • Accession No. Swiss-Prot#: P10244
    NCBI Gene ID: 4605
  • Target Species human
  • Calculated MW 79kd
  • Concentration 1.0mg/ml
  • Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20˚C
Application Details

Western blotting: 1:1000-3000

IHC dilution: 1:50-1:200

Elisa dilution: 1:20000-1:40000

Images
  • B-Myb (Phospho-Thr494/497) Antibody - SAB | Signalway Antibody

    Western blot analysis B-Myb (Phospho-Thr494/497) using Nocodazole treated A549 whole cell lysates

  • B-Myb (Phospho-Thr494/497) Antibody - SAB | Signalway Antibody

    12420 at 1/200 staining Human breast tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

  • B-Myb (Phospho-Thr494/497) Antibody - SAB | Signalway Antibody

    12420 at 1/200 staining Mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

  • B-Myb (Phospho-Thr494/497) Antibody - SAB | Signalway Antibody

    12420 at 1/200 staining Rat brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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