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GSK3β (Phospho-Ser9) Conjugated Antibody

C11002
Review: comment rank 5 pdf
  Catalog No. Package Size Price
C11002-AF350 100ul $379.00
C11002-AF405 100ul $379.00
C11002-AF488 100ul $379.00
C11002-AF594 100ul $379.00
C11002-AF647 100ul $379.00
C11002-AF680 100ul $379.00
C11002-AF750 100ul $399.00
C11002-Biotin 100ul $359.00

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Description
  • Product Name GSK3β (Phospho-Ser9) Conjugated Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Isotype 195995
  • Purification 187533
  • Applications 8462
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of GSK3β only when phosphorylated at serine 9.
  • Immunogen Description Peptide sequence around phosphorylation site of serine 9 (T-T-S(p)-F-A) derived from Human GSK3β.
  • Conjugates Biotin AF350 AF405 AF488 AF594 AF647 AF680 AF750
  • Alternative Names Factor A;GSK-3 beta;Protein kinase GSK-3-beta;kinase GSK-3 beta
  • Accession No. Swiss-Prot#:P49841
    NCBI Gene ID:2932
    NCBI mRNA#:NM_001146156.1
    NCBI Protein#:NP_001139628.1
  • Calculated MW 46
  • Concentration
  • Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 10mg/ml Bovine Serum Albumin, 0.05% Sodium Azide
  • Storage Store at 4˚C in dark for 6 months
Application Details

Biotin conjugated: Working with enzyme-conjugated streptavidin
ELISA: 1: 200 - 1: 1,000
Western Blot: 1: 200 - 1: 1,000
IHC/ICC: 1: 100 - 1: 500
Flow Cytometry: 1: 50 - 1: 250
IF: 1: 100 - 1: 500
AF350 conjugated: most apllications: 1: 50 - 1: 250
AF405 conjugated: most apllications: 1: 50 - 1: 250
AF488 conjugated: most apllications: 1: 50 - 1: 250
AF594 conjugated: most apllications: 1: 50 - 1: 250
AF647 conjugated: most apllications: 1: 50 - 1: 250
AF680 conjugated: Western blot: 1: 100-1,000
AF750 conjugated: Western blot: 1: 100-1,000
desc_detail:Excitation/Emission
Alexa Fluor 350 (AF350): 346nm/442nm
Alexa Fluor 405 (AF405): 401nm/421nm
Alexa Fluor 488 (AF488): 493nm/519nm
Alexa Fluor 594 (AF594): 591nm/614nm
Alexa Fluor 647 (AF647): 651nm/667nm
Alexa Fluor 680 (AF680): 679nm/702nm
Alexa Fluor 750 (AF750): 749nm/775nm

Product Description
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Background
Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin.
    Please let us know if you have published research using #C11002 so that we can cite your reference.
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    Protocol
    Browsing historyclear
    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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