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Raf1 (Phospho-Ser259) Conjugated Antibody

C11006
Review: comment rank 5 pdf
  Catalog No. Package Size Price
C11006-AF350 100ul $379.00
C11006-AF405 100ul $379.00
C11006-AF488 100ul $379.00
C11006-AF594 100ul $379.00
C11006-AF647 100ul $379.00
C11006-AF680 100ul $379.00
C11006-AF750 100ul $399.00
C11006-Biotin 100ul $359.00

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Description
  • Product Name Raf1 (Phospho-Ser259) Conjugated Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of Raf-1 only when phosphorylated at serine 259.
  • Immunogen Description Peptide sequence around phosphorylation site of serine 259 (S-T-S(p)-T-P) derived from Human Raf1.
  • Conjugates Biotin AF350 AF405 AF488 AF594 AF647 AF680 AF750
  • Alternative Names C-RAF;C-Raf;CRAF
  • Accession No. Swiss-Prot#:P04049
    NCBI Gene ID:5894
    NCBI mRNA#:NM_002880.3
    NCBI Protein#:NP _002871.1
  • Calculated MW 73
  • Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 10mg/ml Bovine Serum Albumin, 0.05% Sodium Azide
  • Storage Store at 4˚C in dark for 6 months
Application Details

Biotin conjugated: Working with enzyme-conjugated streptavidin
ELISA: 1: 200 - 1: 1,000
Western Blot: 1: 200 - 1: 1,000
IHC/ICC: 1: 100 - 1: 500
Flow Cytometry: 1: 50 - 1: 250
IF: 1: 100 - 1: 500
AF350 conjugated: most apllications: 1: 50 - 1: 250
AF405 conjugated: most apllications: 1: 50 - 1: 250
AF488 conjugated: most apllications: 1: 50 - 1: 250
AF594 conjugated: most apllications: 1: 50 - 1: 250
AF647 conjugated: most apllications: 1: 50 - 1: 250
AF680 conjugated: Western blot: 1: 100-1,000
AF750 conjugated: Western blot: 1: 100-1,000
desc_detail:Excitation/Emission
Alexa Fluor 350 (AF350): 346nm/442nm
Alexa Fluor 405 (AF405): 401nm/421nm
Alexa Fluor 488 (AF488): 493nm/519nm
Alexa Fluor 594 (AF594): 591nm/614nm
Alexa Fluor 647 (AF647): 651nm/667nm
Alexa Fluor 680 (AF680): 679nm/702nm
Alexa Fluor 750 (AF750): 749nm/775nm

Product Description
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Background
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
    Please let us know if you have published research using #C11006 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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