• Product NameNa+/K+ ATPase Conjugated Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionAmino acids 551-850 of Na+/K+-ATPase α1 of human origin.
• ConjugatesBiotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
• Excitation EmissionAF350: 346nm/442nm
  AF405: 401nm/421nm
  AF488: 493nm/519nm
  AF555: 555nm/565nm
  AF594: 591nm/614nm
  AF647: 651nm/667nm
  AF680: 679nm/702nm
  AF750: 749nm/775nm
• Alternative Names    ATPase Na+/K+ transporting alpha antibody
  adenosinetriphosphatase antibody
  AT1A1_HUMAN antibody
  ATP1A1 antibody
  ATP1A4 antibody
  ATP1AL2 antibody
  ATP1B antibody
  ATP1B1 antibody
  ATPase Na+/K+ transporting alpha 1 polypeptide antibody
  ATPase Na+/K+ transporting alpha 4 polypeptide antibody
  ATPase Na+/K+ transporting beta 1 polypeptide antibody
  ATPase, Na+/K+ transporting, alpha polypeptide-like 2 antibody
  ATPase, Na+/K+ transporting, beta 1 polypeptide antibody
  Beta 1-subunit of Na(+),K(+)-ATPase antibody
  Na(+)/K(+) ATPase alpha-1 subunit antibody
  Na(+)/K(+) ATPase alpha-4 subunit antibody
  Na+, K+ ATPase alpha subunit antibody
  Na+/K+ ATPase 1 antibody
  Na+/K+ ATPase 4 antibody
  Na+/K+ ATPase, alpha-D polypeptide antibody
  Na, K-ATPase beta-1 polypeptide antibody
  Na, K-ATPase, alpha-A catalytic polypeptide antibody
  Na,K-ATPase catalytic subunit alpha-A protein antibody
  Na,K-ATPase subunit alpha-C antibody
  polypeptide-like 2 antibody
  Sodium pump 1 antibody
  sodium pump 4 antibody
  Sodium pump subunit alpha-1 antibody
  sodium pump subunit alpha-4 antibody
  sodium pump subunit beta-1 antibody
  sodium-potassium ATPase catalytic subunit alpha-1 antibody
  sodium-potassium ATPase catalytic subunit alpha-4 antibody
  sodium-potassium ATPase subunit beta 1 (non-catalytic) antibody
  sodium-potassium ATPase, alpha 4 polypeptide antibody
  sodium-potassium-ATPase, alpha 1 polypeptide antibody
  Sodium/potassium transporting ATPase alpha 1 chain antibody
  Sodium/potassium transporting ATPase subunit beta 1 antibody
  sodium/potassium-dependent ATPase beta-1 subunit antibody
  sodium/potassium-transporting ATPase alpha-4 chain antibody
  sodium/potassium-transporting ATPase beta-1 chain antibody
  Sodium/potassium-transporting ATPase subunit alpha-1 antibody
  sodium/potassium-transporting ATPase subunit alpha-4 antibody
  
• Accession No. Swiss-Prot#:P05023
  
• UniprotP05023
• Gene ID476;
• Calculated MW 100-143kDa
• Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
• Storage Store at 4˚C in dark for 6 months

• Suggested Dilution:
  AF350 conjugated: most applications: 1: 50 - 1: 250
  AF405 conjugated: most applications: 1: 50 - 1: 250
  AF488 conjugated: most applications: 1: 50 - 1: 250
  AF555 conjugated: most applications: 1: 50 - 1: 250
  AF594 conjugated: most applications: 1: 50 - 1: 250
  AF647 conjugated: most applications: 1: 50 - 1: 250
  AF680 conjugated: most applications: 1: 50 - 1: 250
  AF750 conjugated: most applications: 1: 50 - 1: 250
  Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1: 50 - 1: 1,000

The ubiquitously expressed sodium/potassium-ATPase (Na+/K+-ATPase) exists as an oligomeric plasma membrane complex that couples the hydrolysis of one molecule of ATP to the importation of three Na+ ions and two K+ ions against their respective electrochemical gradients. As a member of the P-type family of ion motives, Na+/K+-ATPase plays a critical role in maintaining cellular volume, resting membrane potential and Na+-coupled solute transport. Multiple isoforms of three subunits, α, β and γ, comprise the Na+/K+-ATPase oligomer. The α subunit contains the binding sites for ATP and the cations; the glycosylated β subunit ensures correct folding and membrane insertion of the α subunits. The small γ subunit co-localizes with the α subunit in nephron segments, where it increases the affinity of Na+/K+-ATPase for ATP. The β subunit, but not the γ subunit, is essential for normal activity of Na+/K+-ATPase.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting