• Product NameRecombinant Human Erythroferrone(ERFE),partial
• Brief DescriptionRecombinant Protein
• Host SpeciesYeast
• PurificationGreater than 90% as determined by SDS-PAGE.
• Species ReactivityHomo sapiens (Human)
• Target NameERFE
• Alternative Names    Complement C1q tumor necrosis factor-related protein 15 (Myonectin) (C1QTNF15) (CTRP15) (FAM132B)
• Accession No. Uniprot ID: Q4G0M1
• UniprotQ4G0M1
• Gene ID151176;
• Calculated MW 19.5 kDa
• Tag Info N-terminal 6xHis-tagged
• Target Sequence PGPRAPRVEAAFLCRLRRDALVERRALHELGVYYLPDAEGAFRRGPGLNLTSGQYRAPVAGFYALAATLHVALGEPPRRGPPRPRDHLRLLICIQSRCQRNASLEAIMGLESSSELFTISVNGVLYLQMGQWTSVFLDNASGCSLTVRSGSHFSAVLLGV
• Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

• Expression Region: 195-354aa

  Relevance: Iron-regulatory hormone that acts as an erythroid regulator after hemorrhage: produced by erythroblasts following blood loss and mediates suppression of hepcidin expression in the liver, thereby promoting increased iron absorption and mobilization from stores. Promotes lipid uptake into adipocytes and hepatocytes via transcriptional up-regulation of genes involved in fatty acid uptake.

  Storage Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

  Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Associations among Erythroferrone and Biomarkers of Erythropoiesis and Iron Metabolism, and Treatment with Long-Term Erythropoiesis-Stimulating Agents in Patients on Hemodialysis.Honda H., Kobayashi Y., Onuma S., Shibagaki K., Yuza T., Hirao K., Yamamoto T., Tomosugi N., Shibata T.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting