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SARS-CoV-2 (2019-nCoV) Nucleoprotein / NP Antibody, Rabbit MAb

21805
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  Catalog No. Package Size Price
21805-1 50ul $629.00
21805-2 100ul $1039.00

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Description
  • Product Name SARS-CoV-2 (2019-nCoV) Nucleoprotein / NP Antibody, Rabbit MAb
  • Purification Protein A
  • Specificity 2019-nCoV CoV Nucleocapsid Has cross-reactivity in ELISA and WB with SARS-CoV Nucleoprotein / NP Protein .
  • Conjugates Unconjugated
  • Alternative Names Anti-coronavirus NP Antibody; Anti-coronavirus Nucleocapsid Antibody; Anti-coronavirus Nucleoprotein Antibody; Anti-cov np Antibody; Anti-ncov NP Antibody; Anti-NCP-CoV Nucleocapsid Antibody; Anti-novel coronavirus NP Antibody; Anti-novel coronavirus Nucleocapsid Antibody; Anti-novel coronavirus Nucleoprotein Antibody; Anti-np Antibody; Anti-nucleocapsid Antibody; Anti-Nucleoprotein Antibody
  • Formulation 0.2 um filtered solution in PBS
  • Storage This antibody can be stored at 2˚C-8˚C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20˚C to -80˚C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Application Details

WB 1:1000-1:5000
Validated Applications:WB,ELISA,IHC-P,FCM,ICC/IF,IP (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)

Product Description
Preparation Method:This antibody was obtained from a rabbit immunized with purified, recombinant SARS-CoV Nucleoprotein / NP ( NP_828858.1; Met1-Ala422).
Shipping Method:This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Background

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

Regerences

Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6. Li W. et al., 2005, Science. 309 (5742): 1864-8.

    Please let us know if you have published research using #21805 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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