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Enzymes

Histone H2A.X(Phospho-Ser139) Conjugated Antibody #C11268

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Catalog No. Package Size Price Quantity
100ul $399.00
100ul $399.00
100ul $399.00
100ul $399.00
100ul $399.00
100ul $399.00
100ul $399.00
100ul $399.00
100ul $399.00
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Description
  • Product NameHistone H2A.X(Phospho-Ser139) Conjugated Antibody
  • Host SpeciesRabbit
  • ClonalityPolyclonal
  • PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Species ReactivityHu
  • SpecificityThe antibody detects endogenous level of Histone H2A.X only when phosphorylated at serine 139.
  • Immunogen TypePeptide-KLH
  • Immunogen DescriptionPeptide sequence around phosphorylation site of serine 139 (Q-A-S(p)-Q-E) derived from Human Histone H2A.X.
  • ConjugatesBiotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
  • Excitation EmissionAF350: 346nm/442nm AF405: 401nm/421nm AF488: 493nm/519nm AF555: 555nm/565nm AF594: 591nm/614nm AF647: 651nm/667nm AF680: 679nm/702nm AF750: 749nm/775nm
  • Target NameHistone H2A.X
  • ModificationPhospho-Ser139
  • Alternative Names    H2A.X; H2AFX; H2a/x; HIST5-2AX;
  • Accession No. Swiss-Prot: P16104 NCBI Protein: NP_002096.1
  • UniprotP16104
  • Gene ID3014;
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
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Application

WBWestern Blotting

IHCImmunohistochemistry

IFImmunofluorescence

ICCImmunocytochemistry

FCFlow Cytometry

IPImmunoprecipitation

EELISA

DBDot Blotting

ChIPChromatin Immunoprecipitation

GICAGold Immunochromatography Assay

NCNegative Control

Species Reactivity

HuHuman

MsMouse 

RtRat 

Dm Drosophila melanogaster

C Caenorhabditis elegans

MkMonkey

RbRabbit

B Bovine 

D Dog

PPig

HmHamster

ChHm Chinese Hamster 

ChkChicken  

ShpSheep