• Product NameZO-1 Rabbit Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human ZO-1 (NP_003248.3).
• Alternative Names    TJP1;ZO-1;ZO1
• Accession No. Swiss-Prot#:Q07157
  NCBI Gene ID:7082
• UniprotQ07157
• Gene ID7082
• Calculated MW 186kDa/195kDa
• Formulation PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:2000 - 1:5000

  IHC 1:50 - 1:200

  IF   1:50 - 1:200

This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Alternative splicing of this gene results in multiple transcript variants.

• Western blot analysis of extracts of various cell lines, using ZO-1 antibody.
• Western blot analysis of extracts of Rat liver, using ZO-1 antibody.
• Immunohistochemistry of paraffin-embedded Mouse stomach using ZO-1 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
• Immunohistochemistry of paraffin-embedded Rat stomach using ZO-1 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
• Immunofluorescence analysis of MCF7 cells using ZO-1 Rabbit pAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting