• Product NameEGR1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of total EGR1 protein.
• Immunogen TypeRecombinant protein
• Immunogen DescriptionFusion protein corresponding to a region derived from 87-337 amino acids of human early growth response 1early growth response 1
• Target NameEGR1
• Alternative Names    early growth response 1, TIS8, AT225, G0S30, NGFI-A, ZNF225, KROX-24, ZIF-268
• Accession No. Swiss-Prot:P18146
  Gene ID:1958;
• UniprotP18146
• Gene ID1958;
• Concentration 0.1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 58kd
  ELISA: 1:1000-1:5000
  Western blotting: 1:200-1:1000

EGR-1 (Early growth response protein 1) also known as Zif268 (zinc finger protein 225) or NGFI-A (nerve growth factor-induced protein A) is a protein that in humans is encoded by the EGR1 gene. The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site).

• Gel: 8%SDS-PAGE
  Lane1: RAW264.7 cell lysate
  Lane2: NIH/3T3 cell lysate
  Lysates: 50 ug per lane
  Primary antibody: 1/400dilution
  Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
  Exposure time: 2 minutes
  

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting