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Mouse anti-Human CD14, FITC Conjugated mAb

28051
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  Catalog No. Package Size Price
28051-1 50 Tests $279.00

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Description
  • Product Name Mouse anti-Human CD14, FITC Conjugated mAb
  • Host Species Mouse
  • Clonality Monoclonal
  • Clone No. 4AW4
  • Isotype IgG1
  • Applications FC
  • Species Reactivity Hu
  • Conjugates FITC
  • Target Name CD14
  • Formulation Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide and 0.2% (w/v) BSA
  • Storage Store at 4°C. DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.
Application Details

Vol.per.Test: 10 μl/Test

Notice: This reagent has been pre-diluted for use at recommended volume per test in flow cytometry analysis. Typically add 10ul of this reagent to 100μl of experimental sample with 1 X 106 cells per test. Please refer to the detailed protocol when you perform a test.

Images
  • Mouse anti-Human CD14, FITC Conjugated mAb - SAB | Signalway Antibody

    Human peripheral blood monocytes analyzed with FITC CD14 mAb

Background
 4AW4 reacts with CD14, a 53-55 kDa molecule. CD14 is a human high affinity cell-surface receptor for complexes of lipopolysaccharide (LPS-endotoxin) and serum LPS-binding protein (LPB). CD14 antigen has a strong presence on the surface of monocytes/macrophages, is weakly expressed on granulocytes, but not expressed by myeloid progenitor cells. CD14 functions as a receptor for endotoxin; when the monocytes become activated they release cytokines such as TNF, and up-regulate cell surface molecules including adhesion molecules.This clone is cross reactive with non-human primate.
 
1.  Knapp, W et al., eds. (1989) Leucocyte Typing IV : White Cell Differentiation Antigens, Oxford University Press, New York.
2.  Makgoba, MW et al., (1988) Nature 331: 86-88
3.  Boyd. AW et al. (1989) Blood  73: 1896-1903
    Please let us know if you have published research using #28051 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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