• Product NameMER/SKY (Phospho-Tyr749/681) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHuman,Mouse,Rat, Chicken, Zebrafish
• SpecificityThe antibody detects endogenous levels of MER/SKY only when phosphorylated at tyrosine 749/681.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 749/681(K-I-Y(p)-S-G) derived from Human MER/SKY .
• Target NameMER/SKY
• ModificationPhospho
• Alternative Names    C-mer; MERK; MERTK;
• Accession No. Swiss-Prot#: Q12866/Q06418;
  NCBI Gene#: 10461/7301;
  NCBI Protein#: NP_006334.2.
• UniprotQ12866
• Gene ID10461;
• SDS-PAGE MW 97kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100

This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. Mutations in this gene have been associated with disruption of the retinal pigment epithelium (RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa (RP).

Graham D.K., Cell Growth Differ. 5:647-657(1994).
Gal A., Nat. Genet. 26:270-271(2000).
Hillier L.W., Nature 434:724-731(2005).

• Western blot analysis of extracts from 3T3 cells treated with EGF using MER/SKY (Phospho-Tyr749/681) Antibody #11740.The lane on the right is treated with the antigen-specific peptide.
• Immunohistochemical analysis of paraffin-embedded human brain tissue using MER/SKY (Phospho-Tyr749/681) antibody #11740 (left)or the same antibody preincubated with blocking peptide (right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting