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Ephrin B1/B2/B3 (phospho Y324/Y311/Y318) Antibody

11780
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  Catalog No. Package Size Price
11780-1 50ul $189.00
11780-2 100ul $279.00

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Description
  • Product Name Ephrin B1/B2/B3 (phospho Y324/Y311/Y318) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications WB IHC
  • Species Reactivity Hu
  • Specificity The antibody detects endogenous levels of Ephrin B1/B2/B3 only when phosphorylated at tyrosine 324/311/318.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around phosphorylation site of tyrosine 324/311/318 (G-D-Y(p)-G-H) derived from Human Ephrin B1/B2/B3 .
  • Target Name Ephrin B1/B2/B3
  • Modification Phospho
  • Alternative Names EPL2; CEL5-L; EFL-3; EFNB1; ELK-L
  • Accession No. Swiss-Prot#: P98172/P52799/Q15768;
    NCBI Gene#: 1947/1948/1949;
    NCBI Protein#: NP_004420.1.
  • SDS-PAGE MW 46kd
  • Concentration 1.0mg/ml
  • Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20˚C/1 year
Application Details

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images
  • Ephrin B1/B2/B3 (phospho Y324/Y311/Y318) Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts from K562 cells treated with serum using Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody #11780.The lane on the right is treated with the antigen-specific peptide.

  • Ephrin B1/B2/B3 (phospho Y324/Y311/Y318) Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human brain tissue using Ephrin B1/B2/B3 (Phospho-Tyr324) antibody #11780 (left)or the same antibody preincubated with blocking peptide (right).

Background

This gene encodes a member of the ephrin family. The encoded protein is a type I membrane protein and a ligand of Eph-related receptor tyrosine kinases. It may play a role in cell adhesion and function in the development or maintenance of the nervous system.

Beckmann M.P., EMBO J. 13:3757-3762(1994).
Davis S., Science 266:816-819(1994).
Fletcher F.A., Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.

    Please let us know if you have published research using #11780 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
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