• Product NameEGFR(Ab-1172) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total EGFR protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa. 1170~1174 (P-D-Y-Q-Q) derived from Human EGFR.
• Target NameEGFR
• Alternative Names    Receptor tyrosine-protein kinase ErbB-1
• Accession No. Swiss-Prot: P00533
  NCBI Protein: NP_005219.2
• UniprotP00533
• Gene ID1956;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 175kd
  Western blotting: 1:500~1:1000
  

Receptor for EGF, but also for other members of the EGF family, as TGF-a, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.

Noguchi T, et al. (1994) Mol Cell Biol; 14(10): 6674-6682
Doherty JK, et al. (1999) Proc Natl Acad Sci U S A; 96(19): 10869-10874
Kanner SB, et al. (1991) Mol Cell Biol; 11(2): 713-720
Wu TT, et al. (1998) Mol Biol Cell; 9(7): 1661-1674
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• Western blot analysis of extracts from HUVEC cells using EGFR(Ab-1172) Antibody #21213.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting