• Product NameS100A12 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu Mouse Rat
• SpecificityThe antibody detects endogenous level of total S100A12 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human S100A12.
• Target NameS100A12
• Alternative Names    p6; CAGC; CGRP; MRP6; CAAF1
• Accession No. Swiss-Prot:P80511
  NCBI Gene ID:6283
• UniprotP80511
• Gene ID6283;
• SDS-PAGE MW 10KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:2000
  Immunohistochemistry: 1:50 - 1:200
  Immunofluorescence: 1:50 - 1:200

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities.

• Western blot analysis of extracts of various cell lines, using S100A12 antibody.
• Immunohistochemical analysis of paraffin-embedded human thyroid cancer using S100A12 antibody at dilution of 1:200 (400x lens).
• Immunofluorescence analysis of HeLa cell using S100A12 antibody. Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting