• Product NameKi67 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsIHC IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total ki67protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from C-terminal of human ki67.
• Target NameKi67
• Alternative Names    Antigen KI-67; MKI67;
• Accession No. Swiss-Prot: P46013
  NCBI Gene ID: 4288
• UniprotP46013
• Gene ID4288;
• SDS-PAGE MW 360kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• 
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Thought to be required for maintaining cell proliferation.

Mitch Dowsett, J Natl Cancer Inst, Jan 2007; 99: 167 - 170.
Phyllis A. Gimotty, J. Clin. Oncol, Nov 2005; 23: 8048 - 8056.
Tim J. Dudderidge, Clin. Cancer Res., Apr 2005; 11: 2510 - 2517.
Scott F. Sieg, J. Immunol., Nov 2003; 171: 5208.

• Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Ki67antibody #33423.
• Immunofluorescence analysis of COS7 cells, using Ki67antibody #33423.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting