• Product NameP300/CBP Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total P300/CBP protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from human P300/CBP.
• Target NameP300/CBP
• Alternative Names    CREB-binding protein; CREBBP E1A-associated protein p300; EP300; P300 transcription coactivator homolog;
• Accession No. Swiss-Prot: Q09472/Q92793
  NCBI Gene ID: 2033
• UniprotQ09472
• Gene ID2033;
• SDS-PAGE MW 93kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100

Acetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 and FOXO1. Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1 in the presence of EP300. Acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators: NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers.

Gerd A. Blobel, J. Leukoc. Biol., Apr 2002; 71: 545.
Eric Kalkhoven, Hum. Mol. Genet., Feb 2003; 12: 441 - 450.
Wei-fang Shen, Mol. Cell. Biol., Nov 2001; 21: 7509 - 7522.
Frederick C. Nucifora, Science, Mar 2001; 291: 2423.

• Western blot analysis of extracts from COLO205 cells, using P300/CBP antibody #33460.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using P300/CBP antibody #33460.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting