• Product NameFas Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total Fas protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.323~327 (E-N-S-N-F) derived from human Fas .
• Target NameFas
• Alternative Names    FASLG receptor; Apoptosis-mediating surface antigen FAS; Apo-1 antigen
• Accession No. Swiss-Prot: P25445
  NCBI Protein: NP_000034.1
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 40-50kd
  Western blotting: 1:1000
  

Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).

Hahne, M. et al. (1995) Int Immunol 7, 1381-6.
Nagata, S. (1997) Cell 88, 355-65.
Green, D.R. and Ferguson, T.A. (2001) Nat Rev Mol Cell Biol 2, 917-24.

• Western blot analysis of extracts from HepG2, Hela and 293 cells using Fas Antibody #21419.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting