• Product NameKNTC2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IF
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total KNTC2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human KNTC2.
• Target NameKNTC2
• Alternative Names    HEC1; HEC; Highly expressed in cancer; rich in leucine heptad repeats; KNTC2
• Accession No. Swiss-Prot: O14777
  NCBI Gene ID: 10403
• UniprotO14777
• Gene ID10403;
• SDS-PAGE MW 73kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  Immunofluorescence: 1:100~1:500

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.

Chen Y., Mol. Cell. Biol. 17:6049-6056(1997).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Zheng L., Mol. Cell. Biol. 19:5417-5428(1999).

• Western blot analysis of extracts from Jurkat cells, using KNTC2 antibody #33568.
• Immunofluorescence analysis of HUVEC cells, using KNTC2 antibody #33568.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting