• Product NameARSI Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total ARSI protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human ARSI.
• Target NameARSI
• Alternative Names    arylsulfatase I; ASI; EC 3.1.6.-;
• Accession No. Swiss-Prot: Q5FYB1
  NCBI Gene ID: 340075
• UniprotQ5FYB1
• Gene ID340075;
• SDS-PAGE MW 64kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  Immunofluorescence: 1:100~1:500

Displays arylsulfatase activity at neutral pH, when co-expressed with SUMF1; arylsulfatase activity is measured in the secretion medium of retinal cell line, but no activity is recorded when measured in cell extracts.

Sardiello M., Hum. Mol. Genet. 14:3203-3217(2005).

• Western blot analysis of extracts from COS7 cells, using ARSI antibody #34444.
• Immunofluorescence analysis of MCF-7 cells, using ARSI antibody #34444.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting