• Product NameCOMT Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total COMT protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human COMT.
• Target NameCOMT
• Alternative Names    Catechol O-methyltransferase; membrane-bound form; catechol-O-methyltransferase; EC 2.1.1.6; MB-COMT
• Accession No. Swiss-Prot: P21964
  NCBI Gene ID: 1312
• UniprotP21964
• Gene ID1312;
• SDS-PAGE MW 30kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.

Lundstroem K., DNA Cell Biol. 10:181-189(1991).
Bertocci B., Proc. Natl. Acad. Sci. U.S.A. 88:1416-1420(1991).
Tenhunen J., Eur. J. Biochem. 223:1049-1059(1994).

• Western blot analysis of extracts from HepG2 cells and HUVEC cells, using COMT antibody #34554.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting