• Product NameNT5C1A Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total NT5C1A protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human NT5C1A.
• Target NameNT5C1A
• Alternative Names    'nucleotidase; cytosolic IA; 5NT1A; CN-I; CN-IA
• Accession No. Swiss-Prot: Q9BXI3
  NCBI Gene ID: 84618
• UniprotQ9BXI3
• Gene ID84618;
• SDS-PAGE MW 41kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  Immunofluorescence: 1:100~1:500

Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides and has a broad substrate specificity. Helps to regulate adenosine levels in heart during ischemia and hypoxia.

Hunsucker S.A., J. Biol. Chem. 276:10498-10504(2001).
Gregory S.G., Nature 441:315-321(2006).
The MGC Project Team; Genome Res. 14:2121-2127(2004).

• Western blot analysis of extracts from MCF-7 cells and HepG2 cells, using NT5C1A antibody #34634.
• Immunofluorescence analysis of A549 cells, using NT5C1A antibody #34634.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting