• Product NameNDUFA4 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total NDUFA4 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human NDUFA4.
• Target NameNDUFA4
• Alternative Names    CI-MLRQ; complex I-MLRQ; EC 1.6.5.3; EC 1.6.99.3; MLRQ
• Accession No. Swiss-Prot: O00483
  NCBI Gene ID: 4697
• UniprotO00483
• Gene ID4697;
• SDS-PAGE MW 9kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Kim J.W., Biochem. Mol. Biol. Int. 43:669-675(1997).
Kanagarajah D., Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases.
Ebert L., Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.

• Western blot analysis of extracts from HepG2 cells and RAW264.7 cells, using NDUFA4 antibody #34827.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting