• Product NameNLE1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total NLE1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human NLE1.
• Target NameNLE1
• Alternative Names    FLJ10458; Nle; notchless homolog 1;
• Accession No. Swiss-Prot: Q9NVX2
  NCBI Gene ID: 54475
• UniprotQ9NVX2
• Gene ID54475;
• SDS-PAGE MW 48kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Plays a role in regulating Notch activity. Plays a role in regulating the expression of CDKN1A and several members of the Wnt pathway, probably via its effects on Notch activity. Required during embryogenesis for inner mass cell survival By similarity.

Ota T., Nat. Genet. 36:40-45(2004).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Bienvenut W.V.; Submitted (AUG-2005) to UniProtKB.

• Western blot analysis of extracts from COS cells, using NLE1 antibody #34866.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting