• Product NameRB1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total RB1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to residues near the C terminal of human retinoblastoma 1
• Target NameRB1
• Alternative Names    RB; pRb; OSRC; pp110; p105-Rb
• Accession No. Swiss-Prot#: P06400
  NCBI Gene ID: 5925
  Gene Accssion: BC039060
• UniprotP06400
• Gene ID5925;
• SDS-PAGE MW 106kd
• Concentration 2.1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma

• Gel: 6%SDS-PAGE
  Lysate: 40ug A431 cell
  Primary antibody: 1/500 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 5 seconds

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting