Product Detail
Product NameIkB-a(Phospho-Ser32/Ser36) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IHC IF
Species ReactivityHu Ms
SpecificityThe antibody detects endogenous level of IkB-a only when phosphorylated at serine 32/36.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of serine 32/36 (H-D-S(p)-G-L-D-S(p)-M-K) derived from Human IkB-a.
Target NameIkB-a
ConjugateUnconjugated
Other NamesI-kappa-B-alpha; IKBA; NF-kappaB inhibitor alpha; NFKBI; NFKBIA
Accession NoSwiss-Prot: P25963
NCBI Protein: NP_065390.1
Uniprot
P25963
Gene ID
4792;
Concentration1.0mg/ml
FormulationLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
WB 1：500 - 1：1000, IHC 1：100 - 1：200, IF 1：100 - 1：500
If you have published an article using product 11152, please notify us so that we can cite your literature.
et al,Cordycepin suppresses TNF-娄脕-induced NF-娄脢B activation by reducing p65 transcriptional activity, inhibiting I娄脢B娄脕 phosphorylation, and blocking IKK娄脙 ubiquitination.
, (2012),
PMID:
23102662
et al,Downregulated Expression of Solute Carrier Family 26 Member 6 in NRK-52E Cells Attenuates Oxalate-Induced Intracellular Oxidative Stress.In Oxid Med Cell Longev.On 2018 Oct 10 by Jiang H, Gao X et al..PMID:30405874
, (2018),
PMID:
30405874
et al,Inhibition of TPL2 by interferon-α suppresses bladder cancer through activation of PDE4D.In J Exp Clin Cancer Res. On 2018 Nov 27 by Qiang Z, Zhou ZY et al..PMID: 30482227
, (2018),
PMID:
30482227
et al,Indirect effect of PM1 on endothelial cells via InducIng the release of respiratory Inflammatory cytokInes.In Toxicol In Vitro. On 2019 Jun by Tian G, Wang J,et al..al PMID: 30858030
, (2019),
PMID:
30858030
et al,Ajudecumin A from Ajuga ovalifolia var. calantha exhibits anti-inflammatory activity in lipopolysaccharide-activated RAW264.7 murine macrophages and animal models of acute inflammation.In Pharm Biol. On 2018 Dec by Zhang H, Ren QC et al..PMID: 31070535
, (2018),
PMID:
31070535
et al,HMGN2 regulates non-tuberculous mycobacteria survival via modulation of M1 macrophage polarization. In J Cell Mol Med on 2019 Dec by Wang X, Chen S, et al..PMID:31596045
, (2019),
PMID:
31596045
et al,Phytoestrogens protect joints in collagen induced arthritis by increasing IgG glycosylation and reducing osteoclast activation.In Int Immunopharmacol on 2020 Mar 12 by Du N, Song L, et al..PMID:32172207
, (2020),
PMID:
32172207
More
et al,Cordycepin Suppresses TNF-伪-induced NF-魏B Activation by Reducing p65 Transcriptional Activity, Inhibiting I魏B伪 Phosphorylation, and Blocking IKK纬 Ubiquitination.In Int Immunopharmacol on 2012 Dec by Zhenghua Ren, Jianhua Cui,et al..PMID:23102662
, (2012),
PMID: 23102662
et al,Angiotensin II Induces Connective Tissue Growth Factor Expression in Human Hepatic Stellate Cells by a Transforming Growth Factor 尾-Independent Mechanism.In Sci Rep on 2017 Aug 10 by Ao Li Jingyao Zhang,et al..PMID: 28798388
, (2017),
PMID: 28798388
et al,Inhibitory effects of eugenol on RANKL-induced osteoclast formation via attenuation of NF-魏 B and MAPK pathways.In Connect Tissue Res on 2015 Jun by Vishwa Deepak , Abe Kasonga et al..PMID:25405641
, (2015),
PMID: 25405641
et al,Piperine alleviates osteoclast formation through the p38/c鈥怓os/NFATc1 signaling axis.In Biofactors on Nov-Dec 2015 by Vishwa Deepak , Marlena C Kruger et al..PMID: 26627060
, (2015),
PMID: 26627060
et al,Rumen-derived lipopolysaccharide enhances the expression of lingual antimicrobial peptide in mammary glands of dairy cows fed a high-concentrate diet.In BMC Vet Res on 2016 Jun 27 by Di Jin, Guangjun Chang et al..PMID: 27350130
, (2016),
PMID: 27350130
et al,Protodioscin ameliorates fructose-induced renal injury via inhibition of the mitogen activated protein kinase pathway.In Phytomedicine on 2016 Nov 15 by Jinyang Shen, Xiaolin Yang et al..PMID:27765371
, (2016),
PMID: 27765371
et al,Monotropein alleviates secondary liver injury in chronic colitis by regulating TLR4/NF-kB signaling and NLRP3 inflammasome . In Eur J Pharmacol on 2020 Sep 15 by Yonger Chen , Yingyu Lu, et al..PMID:32710952, , (2020)
, (2020),
PMID: 32710952
et al,Cetuximab enhances oridonin-induced apoptosis through mitochondrial pathway and endoplasmic reticulum stress in laryngeal squamous cell
carcinoma cells. In Toxicol In Vitro on 2020 May 12 by Ning Kang, Shijie Cao, et al..PMID:32407876
, (2020),
PMID: 32407876
et al,Vitexin Protects against Dextran Sodium Sulfate-Induced Colitis in Mice and Its Potential Mechanisms. In J Agric Food Chem on 2022 Sep 28 by Jing Zhang, Feilin Liang,et al..PMID: 36124900
, (2022),
PMID: 36124900
et al,Sirtuin 7 Promotes Alcohol-Associated Liver Injury via Modulating Myeloid Cell Chemokine (C-C Motif) Ligand 2 Secretion through the NF-kB Signaling Pathway
, (2025),
PMID:
et al,Sirtuin 7 Promotes Alcohol-Associated Liver Injury via Modulating Myeloid Cell Chemokine (C-C Motif) Ligand 2 Secretion through the NF-κB Signaling Pathway.
, (2025),
PMID: 39746506
Yes
