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Location： Home > Phospho Antibodies > eIF2a(Phospho-Ser51) Antibody

eIF2a(Phospho-Ser51) Antibody#11279

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Product NameeIF2a(Phospho-Ser51) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsIF,WB,IHC,ELISA   

Species ReactivityHuman,Mouse,Rat,Dog,Pig,Fish

SpecificityThe antibody detects endogenous level of eIF2a only when phosphorylated at serine 51.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 51 (E-L-S(p)-R-R) derived from Human eIF2a.

Target NameeIF2a

ConjugateUnconjugated

Other NamesEukaryotic translation initiation factor 2 subunit alpha; EIF-2A;

Accession NoSwiss-Prot: P05198
NCBI Protein: NP_004085.1

Uniprot P05198

Gene ID 1965;

Concentration1.0mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. 

Western blot analysis of extracts from Jurkat cells untreated or treated with H2O2 using eIF2a(Phospho-Ser51) Antibody #11279.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF2α (Phospho-Ser51) Antibody #11279 (left) or the same antibody preincubated with blocking peptide #51279 (right).
Immunofluorescence staining of methanol-fixed Hela cells using eIF2a(Phospho-Ser51) Antibody #11279.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using eIF2α (Phospho-Ser51) Antibody #11279.
Western blot analysis of lysates from 1) KB, 2) MCF-7 cells, (Green) primary antibody was diluted at 1:1000,

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Xavier Saelens, et al. (2001) J. Biol. Chem; 276: 41620 - 41628.
Hiroyuki Kubota, et al. (2003) J. Biol. Chem ; 278: 20457 - 20460.
Shijian Chu, et al. (2006) Am J Physiol Lung Cell Mol Physiol ; 291: L983 - L992.
Eileen Connolly, et al. (2006) Mol. Cell. Biol ; 26: 3955 - 3965.

If you have published an article using product 11279, please notify us so that we can cite your literature.

et al,Mechanisms of impaired pancreatic β?cell function in high?fat diet?induced obese mice: The role of endoplasmic reticulum stress.In Mol Med Rep on 2020 May; by Yi X, Cai X, et al..PMID:32323766 , (2020),
PMID: 32323766
et al,螖2-Troglitazone promotes cytostatic rather than pro-apoptotic effects in breast cancer cells cultured in high serum conditions. In Cell Cycle on 2016 Dec 16 by Audrey Berthe, St茅phane Flament,et al..PMID: 27753533 , (2016),
PMID: 27753533
et al,Suppressing endoplasmic reticulum stress-related autophagy attenuates retinal light injury. In Aging (Albany NY) on 2020 Aug 28 by Jing-Yao Song, Bin Fan,et al..PMID: 32858529 , (2020),
PMID: 32858529
et al,Qing Hua Chang Yin ameliorates chronic colitis in mice by inhibiting PERK–ATF4–CHOP pathway of ER stress and the NF-κB signalling pathway. In Pharm Biolon 2024 Dec by Liya Liu, Youqin Chen,et al..PMID:39034914 , (2024),
PMID: 39034914
et al,Anti-Leukemic Properties of Aplysinopsin Derivative EE-84 Alone and Combined to BH3 Mimetic A-1210477. , (2021),
PMID: 34063867
et al,Nucleus-targeted ruthenium(II) complex triggers immunogenic cell death and sensitizes melanoma to anti-PD-1 therapy by activating cGAS-STING pathway. , (2025),
PMID: 40022761

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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