Product NameFAK(phospho-Tyr576/Tyr577) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of FAK only when phosphorylated at tyrosine 576/577.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 576/tyrosine 577 (S-T-Y(p)-Y(p)-K-A) derived from Human FAK.

Target NameFAK

ConjugateUnconjugated

Other NamesFADK 1; FAK1; PTK2

Accession NoSwiss-Prot: Q05397
NCBI Protein: NP_005598.3

Uniprot Q05397

Gene ID 5747;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Predicted MW: 125kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200