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FGFR4 (Phospho-Tyr642) Antibody#11836

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Product Detail

Product NameFGFR4 (Phospho-Tyr642) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB,ELISA

Species ReactivityHuman, Mouse, Rat

SpecificityThe antibody detects endogenous levels of FGFR4 only when phosphorylated at tyrosine 642.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 642(I-D-Y(p)-Y-K) derived from Human FGFR4 .

Target NameFGFR4

ConjugateUnconjugated

Other NamesFGFR-4; CD_antigen=CD334; Fibroblast growth factor receptor 4; EC=2.7.10.1;

Accession NoSwiss-Prot#: P22455;
NCBI Gene#: 2264;
NCBI Protein#: NP_002002.3.

Uniprot P22455

Gene ID 2264;

Sdspage MW90kd

Concentration1.0mg/ml

FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000

Western blot analysis of extracts from JK cells (Lane 2), using FGFR4 (Phospho-Tyr642) Antibody #11836. The lane on the left is treated with antigen-specific peptide.
Western blot analysis of extracts from Jurkat, using Phospho-FGFR4 (Tyr642) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.

Receptor for acidic fibroblast growth factor. Does not bind to basic fibroblast growth factor. Binds FGF19.

Partanen J.M., EMBO J. 10:1347-1354(1991) [PubMed: 1709094].
Ron D., J. Biol. Chem. 268:5388-5394(1993) [PubMed: 7680645].
Takaishi S.,Biochem. Biophys. Res. Commun. 267:658-662(2000) [PubMed: 10631118].

If you have published an article using product 11836, please notify us so that we can cite your literature.

et al,Fibroblast growth factor 19 is correlated with an unfavorable prognosis and promotes progression by activating fibroblast growth factor receptor 4 in advanced-stage serous ovarian cancer.In Oncol Rep on 2015 Nov by Lingling Hu , Lanxiang Cong.PMID:26323668 , (2015),
PMID: 26323668
et al,Loss of E-cadherin Activates a Targetable IGF1R Pathway in Invasive Lobular Breast Carcinoma. In Mol Cancer Res on 2022 Sep 2 by Ashuvinee Elangovan, Jagmohan Hooda,et al..PMID:35665642 , (2022),
PMID: 35665642
et al,Loss of E-cadherin Induces IGF1R Activation and Reveals a Targetable Pathway in Invasive Lobular Breast Carcinoma. In Mol Cancer Res on 2022 Sep 2 by Ashuvinee Elangovan, Jagmohan Hooda, et al..PMID:35665642 , (2022),
PMID: 35665642
et al,Estrogen regulation and functional role of FGFR4 in estrogen receptor positive breast cancer. In bioRxiv [Preprint] on 2024 Mar 20 by Kai Ding, Lyuqin Chen, et al..PMID:38562741 , (2024),
PMID: 38562741
et al,FGFR4 in endocrine resistance: overexpression and estrogen regulation without direct causative role. , (2025),
PMID: 40097769

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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