Product Detail
Product Namep53(Ab-46) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total p53 protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa. 44~48 (M-L-S-P-D) derived from Human p53.
Target Namep53
ConjugateUnconjugated
Other NamesTumor suppressor p53; Phosphoprotein p53; Antigen NY-CO-13
Accession NoSwiss-Prot: P04637
NCBI Protein: NP_000537.3
Uniprot
P04637
Gene ID
7157;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 53kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from HT29 cells using p53(Ab-46) Antibody #21090.
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over.
Dhavan, R. and Tsai, L.H. (2001) Nat Rev Mol Cell Biol. 2: 749-759.
Patrick, G. N. et al. (1998) J Biol Chem. 273: 24057-24064.
Di Stefano V, et al. (2005) Oncogene. 24(35):5431-5442.
Mayo LD, et al.(2005) J Biol Chem. 280(28):25953-25959.
Wang L, et al. (2005) Oncogene. 24(18): 3020-3027.
If you have published an article using product 21090, please notify us so that we can cite your literature.
et al,Xanthatin Induces Cell Cycle Arrest at G2/M Checkpoint and Apoptosis via Disrupting NF-娄脢B Pathway in A549 Non-Small-Cell Lung Cancer Cells.
, (2012),
PMID:
22450683
et al,Nordihydroguaiaretic acid inhibits growth of cervical cancer SiHa cells by up-regulating p21.In Oncol Lett on 2011 Jan by Peng Gao, Fei
Zhai,et al..PMID:22870140
, (2011),
PMID:
22870140
Yes
