Product Detail
Product NameFLT3(Ab-591) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IF
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total FLT3 protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.589~593 (Y-F-Y-V-D) derived from Human FLT3.
Target NameFLT3
ConjugateUnconjugated
Other NamesCD135; FLK-2; FLT-3; FLT3; STK-1
Accession NoSwiss-Prot: P36888
NCBI Protein: NP_004110.2
Uniprot
P36888
Gene ID
2322;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 130 160 kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from 293 and MCF cells using FLT3(Ab-591) Antibody #21187.
Immunofluorescence staining of methanol-fixed Hela cells using FLT3(Ab-591) Antibody #21187.
FLT3 encodes a class III receptor tyrosine kinase that regulates hematopoiesis. The receptor consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, and a cytoplasmic kinase domain split into two parts by a kinase-insert domain. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.
Sekine S et,al. (2008) J Immunol. ;180(12):8126-34
Pratz K et,al. (2008) Leuk Lymphoma. 2008 ;49(5):852-63.
Al Shaer L et,al. (2008) Br J Haematol. 141(4):483-93.
If you have published an article using product 21187, please notify us so that we can cite your literature.
et al,Versatile Immunosensor Using a Quantum Dot Coated Silica Nanosphere as a Label for Signal Amplification.
, (2010),
PMID:
20597496
Yes
