Product Detail
Product NameHistone H3 (Ab-27) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total Histone H3 protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.26-30(A-R-K-S-A) derived from Human Histone H3.
Target NameHistone H3
ConjugateUnconjugated
Other NamesH3/A; H3FA
Accession NoSwiss-Prot: P68431
NCBI Gene ID: 8350
NCBI mRNA: NM_003529.2
NCBI Protein: NP_003520.1
Uniprot
P68431
Gene ID
8350;8351;8352;8353;8354;8355;8356;8357;8358;8968;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C
Application Details
Predicted MW: 17kd
Western blotting: 1:500~1:1000
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Choi H.S., Choi B.Y., Cho Y.-Y., Zhu F., Bode A.M., Dong Z.J. Biol. Chem. 280:13545-13553(2005)
Tan M., Luo H., Lee S., Jin F., Yang J.S., Montellier E., Buchou T., Cheng Z., Rousseaux S., Rajagopal N., Lu Z., Ye Z., Zhu Q., Wysocka J., Ye Y., Khochbin S., Ren B., Zhao Y.
Cell 146:1016-1028(2011)
If you have published an article using product 21689, please notify us so that we can cite your literature.
et al,Inhibitory effects of Salvianolic acid B on apoptosis of Schwann cells and its mechanism induced by intermittent high glucose
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et al,The antioxidant peptide SS31 prevents oxidative stress, downregulates CD36 and improves renal function in diabetic nephropathy.In Nephrol Dial Transplant. On 2018 Nov by Hou Y, Shi Y et al..PMID: 30388276
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et al,Human umbilical cord mesenchymal stem cell exosomes enhance angiogenesis through the Wnt4/尾鈥恈atenin pathway銆侷n Stem Cells Transl Med on 2015 May by Bin Zhang , Xiaodan Wu et al..PMID:25824139
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et al,Human umbilical cord mesenchymal stem cell exosomes enhance angiogenesis through the Wnt4/β-catenin pathway. In Stem Cells Transl Med on 2015 May by Bin Zhang, Xiaodan Wu, et al..PMID: 25824139
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et al,EFFECTS OF GLUCOSE POLYMER INGESTION ON HIGH-INTENSITY INTERMITTENT CYCLING
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PMID:
Yes
