Product Detail
Product NameIL1B Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were purified by affinity purification using immunogen.
ApplicationsWB,IF
Species ReactivityHuman,Mouse,Rat
SpecificityThe antibody detects endogenous level of total IL1B protein.
Immunogen TypeRecombinant Protein
Immunogen DescRecombinant protein of human IL1B.
Target NameIL1B
ConjugateUnconjugated
Other NamesIL1B; IL-1; IL1-BETA; IL1F2; IL1beta
Accession NoSwiss-Prot:P01584
NCBI Gene ID:3553
Uniprot
P01584
Gene ID
3553;
Sdspage MW31KD
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
WB 1:500 - 1:2000
IF 1:50 - 1:200
Western blot analysis of extracts of mouse liver, using IL1 beta at 1:1000 dilution.
Immunofluorescence analysis of THP-1 cells using IL1 beta Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse lung using IL1 beta Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation. PA700, PA28, and PA200 are three major protein Interleukin-1beta (IL-1beta), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses (1). It is produced primarily by activated monocytes and macrophages. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets (2-6). Human IL-1beta is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form (7,8). Detection of the 17 kDa mature form of IL-1beta is a good indicator of caspase-1 activity.
If you have published an article using product 32165, please notify us so that we can cite your literature.
et al,Targeting the AKT-P53/CREB pathway with epicatechin for improved prognosis of traumatic brain injury
, (2023),
PMID:
et al,Poly-(ADP-ribose) polymerases inhibition by olaparib attenuates activities of the NLRP3 inflammasome and of NF-κB in THP-1 monocytes
, (2024),
PMID:
et al,The role of FTO in m6A RNA methylation and immune regulation in Staphylococcus aureus infection-related osteomyelitis.
, (2025),
PMID:
39980685
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