Product Detail
Product Name4E-BP1 (Ab-64) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ApplicationsWB IHC
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous levels of total 4E-BP1 protein.
Immunogen TypePeptide
Immunogen DescSynthesized non-phosphopeptide derived from human 4E-BP1 around the phosphorylation site of serine 65 (R-N-S(p)-P-V).
Target Name4E-BP1
ConjugateUnconjugated
Other Names4EBP1; EIF4EBP1; P/OKCL.6; PHAS-1;
Accession NoSwiss-Prot: Q13541
NCBI Gene ID: 1978
Uniprot
Q13541
Gene ID
1978;
Sdspage MW15kd
Concentration1.0mg/ml
FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Jurkat cells, treated with Insulin (0.01U/ml, 15mins), using 4E-BP1 (Ab-64) antibody #33127.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using 4E-BP1 (Ab-64) antibody #33127.
Regulates eIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Anne-Claude Gingras, Genes & Dev., Jun 1999; 13: 1422.
Guangming Liu, J. Biol. Chem., Mar 2002; 277: 8810 - 8816.
Ya-Ping Jiang, J. Biol. Chem., Mar 2001; 276: 10943 - 10951.
If you have published an article using product 33127, please notify us so that we can cite your literature.
et al,Monotropein inhibits epithelial-mesenchymal transition in chronic colitis via the mTOR/P70S6K pathway.
, (2025),
PMID:
40041493
Yes
