Product Detail
Product NamePCNA Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ApplicationsWB IHC
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous levels of total PCNA protein.
Immunogen TypePeptide
Immunogen DescSynthesized peptide derived from human PCNA.
Target NamePCNA
ConjugateUnconjugated
Other Namescyclin; proliferating cell nuclear antigen;
Accession NoSwiss-Prot: P12004
NCBI Gene ID: 5111
Uniprot
P12004
Gene ID
5111;
Sdspage MW36kd
Concentration1.0mg/ml
FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PCNA antibody #33468.
Western blot analysis of extracts from HepG2 cells, using PCNA antibody #33468.
Western blot analysis of extracts from Hela cells (Lane 2), colo cells(Lane 3), HepG2 cells (Lane 4) and 293 cells (Lane 5) using PCNA antiobdy #33468. The lane on the left is treated with synthesized peptide.
Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
Anje Sporbert, Nucleic Acids Res., Jun 2005; 33: 3521 - 3528.
Sean C. Gehen, Am J Physiol Lung Cell Mol Physiol, Mar 2007; 292: L716 - L724.
Basavaraj Bagewadi, J. Virol., Nov 2004; 78: 11890 - 11903.
T Iida, I Suetake, Genes Cells, Oct 2002; 7: 997 -
If you have published an article using product 33468, please notify us so that we can cite your literature.
et al,Eurycomalactone switched hepatocellular carcinoma cells into quiescence through 5'tRFAla/DVL/β-catenin pathway inhibition.
, (2025),
PMID:
40128187
Yes
