Product Detail
Product NameRecombinant Human G1,S-specific cyclin-D1(CCND1)
Host SpeciesE.coli
PurificationGreater than 90% as determined by SDS-PAGE.
Immunogen DescExpression Region:1-295aa
Sequence Info:Full Length
Other NamesB-cell lymphoma 1 protein ;BCL-1BCL-1 oncogene;PRAD1 oncogene
Accession NoP24385
Uniprot
P24385
Gene ID
595;
Calculated MW35.88 kDa
FormulationLyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1%Mannitol.
StorageThe shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20˚C,-80˚C. The shelf life of lyophilized form is 12 months at -20˚C,-80˚C.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4˚C for up to one week.
Tag InfoN-His
Application Details
Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits mbers of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1,S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB,E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1,CDK4,CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.
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