• Product NamePNPase antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IHC IF
• Species ReactivityHu
• Immunogen TypeRecombinant protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 753 of PNPase
• Target NamePNPase
• Accession No. Swiss-Prot:Q8TCS8
  Gene ID:87178
• UniprotQ8TCS8
• Gene ID87178;
• Concentration 1mg/ml
• Formulation Supplied in 1XPBS, 1%BSA, 20% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 86kd
  Western blotting: 1:500-1:3000
  Immunohistochemistry: 1:100-1:250
  Immunofluorescence: 1:100-1:200
  

PNPT1 is a subunit of the exosome complex, which is involved in 3-prime-to-5-prime exoribonuclease activity for RNA processing and degradation (Raijmakers et al., 2002 [PubMed 12419256]).[supplied by OMIM]

• Sample (30 ug of whole cell lysate)
  A: A431
  7.5% SDS PAGE
  Primary antibody diluted at 1: 1000
• Immunohistochemical analysis of paraffin-embedded Colon ca, using PNPase antibody at 1: 250 dilution.
• Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PNPase antibody (Green) at 1: 500 dilution and alpha-tubulin antibody (Red) at 1: 2500.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting