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MyoD(Phospho-Ser200) Antibody

11077
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  Catalog No. Package Size Price
11077-1 50ul $189.00
11077-2 100ul $279.00

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Description
  • Product Name MyoD(Phospho-Ser200) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications WB
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of MyoD only when phosphorylated at serine 200.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around phosphorylation site of serine 200 (A-S-S(p)-P-R) derived from Human MyoD.
  • Target Name MyoD
  • Modification Phospho
  • Alternative Names MYF3; MYOD; MYOD1
  • Accession No. Swiss-Prot: P15172
    NCBI Protein: NP _002469.2
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 40kd
Western blotting: 1:500~1:1000

Images
  • MyoD(Phospho-Ser200) Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts from C2C12 and 3T3 cells untreated or treated with EGF using MyoD (Phospho-Ser200) Antibody #11077.

Background

MyoD encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis.

Emmanuel G, et al. J. Biol. Chem., Jun 2000; 275: 18767 - 18776
Tintignac LA, et al. Mol Cell Biol. 2004 Feb; 24(4): 1809-1821.
Kitzmann M, et a. Mol Cell Biol. 1999 Apr; 19(4): 3167-3176.

    Please let us know if you have published research using #11077 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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