• Product NameNDUFB5 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IHC IF
• Species ReactivityHu
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide contain a sequence corresponding to a region within amino acids 126 and 189 of human NDUFB5
• Target NameNDUFB5
• Accession No. Swiss-Prot:O43674
  Gene ID:4711
• UniprotO43674
• Gene ID4711;
• Concentration 0.4mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 22kd
  Western blotting: 1:500-1:3000
  Immunohistochemistry: 1:50-1:500
  Immunofluorescence: 1:100-1:200
  

The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [provided by RefSeq]

• Sample (30 ug of whole cell lysate)
  A: Molt-4
  12% SDS PAGE
  Primary antibody diluted at 1: 1000
• Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using NDUFB5 antibody at 1: 500 dilution.
• Immunofluorescence analysis of methanol-fixed HeLa, using NDUFB5 antibody at 1: 200 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting